| Pos | Rider | Nat | Team | Motorcycle | Time | Gap | To 1st |
| 1. | Alex Debon | E | Aeropuerto-Castello-Bluse | Aprilia | 1:36.116 | | |
| 2. | Marco Simoncelli | I | Metis Gilera | Gilera | 1:36.450 | 0.334 | 0.334 |
| 3. | Hector Barbera | E | Pepe World Team | Aprilia | 1:36.470 | 0.020 | 0.354 |
| 4. | Ratthapark Wilairot | T | Thai Honda PTT SAG | Honda | 1:36.563 | 0.093 | 0.447 |
| 5. | Hiroshi Aoyama | J | Scot Racing Team 250cc | Honda | 1:36.656 | 0.093 | 0.540 |
| 6. | Thomas Luthi | CH | Emmi - Caffe Latte | Aprilia | 1:36.985 | 0.329 | 0.869 |
| 7. | Karel Abraham | CZ | Cardion AB Motoracing | Aprilia | 1:37.271 | 0.286 | 1.155 |
| 8. | Mattia Pasini | I | Paddock GP Racing Team | Aprilia | 1:37.278 | 0.007 | 1.162 |
| 9. | Alvaro Bautista | E | Mapfre Aspar Team | Aprilia | 1:37.303 | 0.025 | 1.187 |
| 10. | Hector Faubel | E | Honda SAG | Honda | 1:37.318 | 0.015 | 1.202 |
| 11. | Raffaele De Rosa | I | Scot Racing Team 250cc | Honda | 1:37.365 | 0.047 | 1.249 |
| 12. | Alex Baldolini | I | WTR San Marino Team | Aprilia | 1:37.577 | 0.212 | 1.461 |
| 13. | Jules Cluzel | F | Matteoni Racing | Aprilia | 1:37.749 | 0.172 | 1.633 |
| 14. | Roberto Locatelli | I | Metis Gilera | Gilera | 1:37.811 | 0.062 | 1.695 |
| 15. | Mike Di Meglio | F | Mapfre Aspar Team 250cc | Aprilia | 1:37.958 | 0.147 | 1.842 |
| 16. | Shoya Tomizawa | J | CIP Moto - GP250 | Honda | 1:38.085 | 0.127 | 1.969 |
| 17. | Axel Pons | E | Pepe World Team | Aprilia | 1:38.126 | 0.041 | 2.010 |
| 18. | Lukas Pesek | CZ | Auto Kelly - CP | Aprilia | 1:38.291 | 0.165 | 2.175 |
| 19. | Balazs Nemeth | H | Balatonring Team | Aprilia | 1:38.737 | 0.446 | 2.621 |
| 20. | Shuhei Aoyama | J | Racing Team Germany | Honda | 1:38.795 | 0.058 | 2.679 |
| 21. | Valentin Debise | F | CIP Moto - GP250 | Honda | 1:39.283 | 0.488 | 3.167 |
| 22. | Vladimir Leonov | RUS | Viessmann Kiefer Racing | Aprilia | 1:40.371 | 1.088 | 4.255 |
| 23. | Bastien Chesaux | CH | Matteoni Racing | Aprilia | 1:41.605 | 1.234 | 5.489 |
| 24. | Imre Toth | H | Team Toth Aprilia | Aprilia | 1:42.418 | 0.813 | 6.302 |