| Pos | Rider | Nat | Team | Motorcycle | Time | Gap | To 1st |
| 1. | Mike Di Meglio | F | Mapfre Aspar Team 250cc | Aprilia | 1:36.356 | | |
| 2. | Alex Debon | E | Aeropuerto-Castello-Bluse | Aprilia | 1:36.568 | 0.212 | 0.212 |
| 3. | Hector Barbera | E | Pepe World Team | Aprilia | 1:36.583 | 0.015 | 0.227 |
| 4. | Hiroshi Aoyama | J | Scot Racing Team 250cc | Honda | 1:36.635 | 0.052 | 0.279 |
| 5. | Raffaele De Rosa | I | Scot Racing Team 250cc | Honda | 1:36.815 | 0.180 | 0.459 |
| 6. | Alvaro Bautista | E | Mapfre Aspar Team | Aprilia | 1:36.957 | 0.142 | 0.601 |
| 7. | Thomas Luthi | CH | Emmi - Caffe Latte | Aprilia | 1:36.959 | 0.002 | 0.603 |
| 8. | Marco Simoncelli | I | Metis Gilera | Gilera | 1:37.202 | 0.243 | 0.846 |
| 9. | Mattia Pasini | I | Paddock GP Racing Team | Aprilia | 1:37.303 | 0.101 | 0.947 |
| 10. | Ratthapark Wilairot | T | Thai Honda PTT SAG | Honda | 1:37.366 | 0.063 | 1.010 |
| 11. | Shoya Tomizawa | J | CIP Moto - GP250 | Honda | 1:37.452 | 0.086 | 1.096 |
| 12. | Hector Faubel | E | Honda SAG | Honda | 1:37.518 | 0.066 | 1.162 |
| 13. | Alex Baldolini | I | WTR San Marino Team | Aprilia | 1:37.593 | 0.075 | 1.237 |
| 14. | Karel Abraham | CZ | Cardion AB Motoracing | Aprilia | 1:37.618 | 0.025 | 1.262 |
| 15. | Roberto Locatelli | I | Metis Gilera | Gilera | 1:37.751 | 0.133 | 1.395 |
| 16. | Lukas Pesek | CZ | Auto Kelly - CP | Aprilia | 1:38.154 | 0.403 | 1.798 |
| 17. | Axel Pons | E | Pepe World Team | Aprilia | 1:38.459 | 0.305 | 2.103 |
| 18. | Jules Cluzel | F | Matteoni Racing | Aprilia | 1:38.692 | 0.233 | 2.336 |
| 19. | Balazs Nemeth | H | Balatonring Team | Aprilia | 1:38.856 | 0.164 | 2.500 |
| 20. | Valentin Debise | F | CIP Moto - GP250 | Honda | 1:38.948 | 0.092 | 2.592 |
| 21. | Shuhei Aoyama | J | Racing Team Germany | Honda | 1:39.141 | 0.193 | 2.785 |
| 22. | Bastien Chesaux | CH | Matteoni Racing | Aprilia | 1:40.843 | 1.702 | 4.487 |
| 23. | Vladimir Leonov | RUS | Viessmann Kiefer Racing | Aprilia | 1:41.115 | 0.272 | 4.759 |
| 24. | William Dunlop | IRL | Bigman Racing | Honda | 1:42.910 | 1.795 | 6.554 |