Repsol Honda riders Marc Marquez and Dani Pedrosa will have their first taste of the Japanese manufacturer’s 2016 bike in testing at Misano on Thursday.
The new prototype was ridden for the first time on Wednesday by HRC test rider Hiroshi Aoyama, with the team's race pairing set to have their turn having spent the first day of testing fine-tuning the 2015 bike.
"The chassis is completely new, while the engine is still in the current 2015 configuration," Honda team principal Livio Suppo told GPOne.com.
"It is only the first test and will show us if the direction taken is the right one.
"In addition, here we will race in two months, so there is the chance for Marc and Dani to learn about the newly resurfaced track," he added.
Ducati hoping to make gains
Also testing at Misano is the works Ducati team, which is hoping to use the running at Misano to recover some of the ground it has conceded to Honda and Yamaha in the last two races.
"In these tests at Misano, we aim to understand how the gap has been created between us and the best teams," said Ducati's Davide Tardozzi.
"At the beginning of the season we were competitive and now we have lost touch. We are doing tests of various kinds; we absolutely must catch up."
Suzuki and the satellite Pramac Ducati squad are also in action in Italy, with the works Yamaha squad the most notable absentee from the test.